Involvement of the Enteroaggregative Escherichia coli Plasmid-Encoded Toxin in Causing Human Intestinal Damage
نویسندگان
چکیده
منابع مشابه
Involvement of the enteroaggregative Escherichia coli plasmid-encoded toxin in causing human intestinal damage.
Enteroaggregative Escherichia coli (EAEC) strains have been shown to adhere to human intestinal tissue in an in vitro organ culture (IVOC) model, and certain strains manifest mucosal toxicity. We have recently described the EAEC plasmid-encoded toxin (Pet), a member of a specific serine protease subclass of the autotransporter proteins. When injected into rat ileal loops, Pet both elicited flui...
متن کاملIntracellular expression of the plasmid-encoded toxin from enteroaggregative Escherichia coli.
The plasmid-encoded toxin (Pet) from enteroaggregative Escherichia coli is a serine protease autotransporter that acts as an enterotoxin and cytotoxin. When applied to epithelial cells in culture, purified toxin induces cell elongation and rounding, followed by exfoliation of cells from the substratum. These effects are accompanied by loss of actin stress fibers and electrophysiologic changes. ...
متن کاملPlasmid-encoded toxin of enteroaggregative Escherichia coli is internalized by epithelial cells.
We have previously described a 104-kDa protein termed Pet (for plasmid-encoded toxin) secreted by some strains of enteroaggregative Escherichia coli (EAEC). Through an unknown mechanism, this toxin (i) raises transepithelial short-circuit current (Isc) and decreases the electrical resistance of rat jejunum mounted in the Ussing chamber, (ii) causes cytoskeletal alterations in HEp-2 cells and HT...
متن کاملAtypical Enteropathogenic Escherichia coli Secretes Plasmid Encoded Toxin
Plasmid encoded toxin (Pet) is a serine protease originally described in enteroaggregative Escherichia coli (EAEC) prototype strain 042 whose entire characterization was essentially obtained from studies performed with the purified toxin. Here we show that Pet is not exclusive to EAEC. Atypical enteropathogenic Escherichia coli (aEPEC) strains, isolated from diarrhea cases, express Pet and its ...
متن کاملPlasmid encoded enterotoxin (Pet) gene in enteroaggregative Escherichia coli isolated from sporadic diarrhea cases.
(2). The aggregative adherence of EAggEC was due to the presence of aggregative adherence fimbriae (AAF), AAF/ 1 and AAF/lI, whose expression lS positively controlled by the aggR gene (2)・ This aggR gene is present in many EAggECs, but some aggR-positive E. coli isolates have no AAFs (3,4). EAggEC was found to produce EAggEC heat-stable enterotoxin (EASTl encoded by astA, molecular weight 4.1 k...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1999
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.67.10.5338-5344.1999